| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.078200 |
| Chromosome: | chromosome 9 |
| Location: | 4425782 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g400553 | TOR1 | (1 of 1) K07203 - FKBP12-rapamycin complex-associated protein (FRAP); Target of rapamycin 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTGGAGGTGCTCATCACCGCCGCCGCCCGCCTCATCATGCCCTACGTC |
| Internal bar code: | GTGTGGTAAAAGGACGATCGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 118 |
| LEAP-Seq percent confirming: | 40.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCTGCCCCTCATCTTCCCT |
| Suggested primer 2: | GGAAACCAAACCCGAAGTGC |