| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.078221 |
| Chromosome: | chromosome 10 |
| Location: | 4999639 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g454350 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGCTTTCTTATGTGTGTGCGCTGTGTATGGCACTGATGTCAGCTTGGG |
| Internal bar code: | CCATCGACTTTCCCCGGTTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2027 |
| LEAP-Seq percent confirming: | 62.5 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAGTGCTCGAATGTTGCA |
| Suggested primer 2: | TTCTGCACACCATAACGCCT |