| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.078228 |
| Chromosome: | chromosome 12 |
| Location: | 5705390 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g532151 | ERM7 | (1 of 13) PTHR13018//PTHR13018:SF5 - PROBABLE MEMBRANE PROTEIN DUF221-RELATED // PHOSPHATE METABOLISM PROTEIN 7; ERD4-related membrane protein | 3'UTR |
| Cre12.g532200 | AOA2 | Putative alcohol O-acetyltransferase; (1 of 4) 2.3.1.84 - Alcohol O-acetyltransferase / AATASE | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGGCCAGTTTCCCATGACTGGCCAACTAACACTCGCCCAGTCGCATGC |
| Internal bar code: | TCCCATATCGTTATTGTAGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1740 |
| LEAP-Seq percent confirming: | 88.8889 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGAGTGAGTGCCTGGTGTC |
| Suggested primer 2: | CGAATAGCCTTTCAGCACGC |