| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.078228 |
| Chromosome: | chromosome 12 |
| Location: | 5705412 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g532151 | ERM7 | (1 of 13) PTHR13018//PTHR13018:SF5 - PROBABLE MEMBRANE PROTEIN DUF221-RELATED // PHOSPHATE METABOLISM PROTEIN 7; ERD4-related membrane protein | 3'UTR |
| Cre12.g532200 | AOA2 | Putative alcohol O-acetyltransferase; (1 of 4) 2.3.1.84 - Alcohol O-acetyltransferase / AATASE | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAGTTATTATTATTTAGCGCTTCTAGCGTCACCCGTGAGCCCCGGGGGT |
| Internal bar code: | TCCCATATCGTTATTGTAGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5170 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 43 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAATAGCCTTTCAGCACGC |
| Suggested primer 2: | TTGAGTGAGTGCCTGGTGTC |