| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.078234 |
| Chromosome: | chromosome 12 |
| Location: | 5482115 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g529950 | EIF4G1,EIF4G | (1 of 2) K03260 - translation initiation factor 4G (EIF4G); Eukaryotic translation initiation factor 4, gamma subunit | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCATCGGCAGGGGGGGGACCTGGGGACTGGGAAACTGAAAAGGTGAGT |
| Internal bar code: | ACAGTAGCAGGGTATTTGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1232 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCATGGCCAGTACCAACT |
| Suggested primer 2: | GAAGCTCGACAAGGTGGTGA |