| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.078252 |
| Chromosome: | chromosome 7 |
| Location: | 2645106 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g330500 | (1 of 5) PF04232 - Stage V sporulation protein S (SpoVS) (SpoVS) | 5'UTR | |
| Cre07.g330550 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTGCCAGGTTCACCTACTGAGCGACACCGGGACTGAAAAGTGGAAGTC |
| Internal bar code: | TCCACTCGATTACCCAGCAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1119 |
| LEAP-Seq percent confirming: | 57.1429 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTGGGGCATCACGATAGG |
| Suggested primer 2: | AGAATTCCGTGCACAGCAGA |