| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.078253 |
| Chromosome: | chromosome 9 |
| Location: | 6173368 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g413150 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAAAGGGTGGTGGAAATAGATGCGTCAATCGGTCGTGAGCAACCAGGG |
| Internal bar code: | TACGTTTATTTCAATACTAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 377 |
| LEAP-Seq percent confirming: | 93.75 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGACTGCCCCCACGTATA |
| Suggested primer 2: | TCCCTCGTTCACTCTTGTGC |