Insertion junction: CLIP2.078264_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.078264
Chromosome:chromosome 12
Location:6308901
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACCAGCGGCAATGGACCCCGACCGTTTAGTGGTCCCACAGGGGCACCCG
Internal bar code:TACTGGGGTTCACTAACCAACC

Confirmation - LEAP-Seq

LEAP-Seq distance:1967
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:37
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:37

Suggested primers for confirmation by PCR

Suggested primer 1:GATCGAGTAGGGCCACATGG
Suggested primer 2:CATTGTATTGAAGGCGCCGG