| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.078281 |
| Chromosome: | chromosome 13 |
| Location: | 577442 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g565450 | PURB,PURB1 | Adenylosuccinate lyase; (1 of 1) K01756 - adenylosuccinate lyase (purB, ADSL) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGTGACACGAGTACGCGAGCAGCAGCAGAGGGACGTTGACGGCGAACAA |
| Internal bar code: | ATGCGGGCCGCGGGTTCGTAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3284 |
| LEAP-Seq percent confirming: | 97.5 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAAGTAGTATTTGCGGCGG |
| Suggested primer 2: | CGTGTGCGTTTTTGCCTGTA |