| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.078283 |
| Chromosome: | chromosome 3 |
| Location: | 2561303 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g159900 | (1 of 1) K07179 - RIO kinase 2 (RIOK2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGAGCATGTGGTAGTCAGAAAGCAGGTACATGCGCGATGCAGACAGCAT |
| Internal bar code: | TTTTCTACGGGCTACACTCACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 837 |
| LEAP-Seq percent confirming: | 70.6667 |
| LEAP-Seq n confirming: | 53 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 75 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTAGCGCATTGTGGAAGCA |
| Suggested primer 2: | GGATGAAGACGAGGAGAGCG |