Insertion junction: CLIP2.078310_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.078310
Chromosome:chromosome 7
Location:2462819
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g329250 Putative metallochaperone; (1 of 1) PF02492//PF07683//PF13414 - CobW/HypB/UreG, nucleotide-binding domain (cobW) // Cobalamin synthesis protein cobW C-terminal domain (CobW_C) // TPR repeat (TPR_11) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCCGATTGAATGACCATGAAGCAATTTGGAGTTTTGTCCTCGCGGCTGG
Internal bar code:TACACGTGTTCCGTCGATCGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1992
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:52
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:52

Suggested primers for confirmation by PCR

Suggested primer 1:AGTGCTGATGGTGGTAGTGC
Suggested primer 2:AAAAACACAGCCATCGCCAC