Insertion junction: CLIP2.078312_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.078312
Chromosome:chromosome 13
Location:1349723
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g571450 (1 of 2) K17278 - membrane-associated progesterone receptor component (PGRMC1_2) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCTGGGCTAATACTCGCCGCCGCAATGTCGCACACGTTCACCGTGGAGG
Internal bar code:TGCTGTAACTGGAAGGACTTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1245
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:ACGCGCCTAAAGAGTATCGG
Suggested primer 2:GCGCACCTTCTTGCAAGAAA