Insertion junction: CLIP2.078332_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.078332
Chromosome:chromosome 6
Location:3109302
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g275400 (1 of 1) PTHR10331 - T COMPLEX PROTEIN 10 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGCCATCCAATGATCCAATGTTACAACTCCGCACTCCCGTCGTGCCCGC
Internal bar code:TATCGCTAGACAGGGTAGAATC

Confirmation - LEAP-Seq

LEAP-Seq distance:2013
LEAP-Seq percent confirming:17.8571
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:46
LEAP-Seq n unique pos:56

Suggested primers for confirmation by PCR

Suggested primer 1:GGGTGACTGACTGTGTCCTG
Suggested primer 2:AGGAGGCCATAGCGAAATCG