Insertion junction: CLIP2.078340_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.078340
Chromosome:chromosome 10
Location:2723933
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g437850 SAE1,CBP20 CAP-binding protein 20; (1 of 1) K12883 - nuclear cap-binding protein subunit 2 (NCBP2, CBP20) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCATCAGACGGTGCGGCGAAGTGGCCCGGGACGAGAGTGAAGTCACAT
Internal bar code:AAAGGTAACATGGAACAGGCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:3668
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:40
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:40

Suggested primers for confirmation by PCR

Suggested primer 1:CACAGCAAGGAGGAGGTGAG
Suggested primer 2:TGCCCCAATCAAGCGATCTT