| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.078350 |
| Chromosome: | chromosome 1 |
| Location: | 5366396 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g037900 | (1 of 2) K03257 - translation initiation factor 4A (EIF4A) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCGGCCTTAACCCAGCTCCACCCCAGGACTCCAGCCTAGGCCTGATTG |
| Internal bar code: | GTCCTCAGAGATTTCGACCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 174 |
| LEAP-Seq percent confirming: | 72.7273 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACATCGTACATGTTGCGGC |
| Suggested primer 2: | ATACGCCCCAAAACCTCCTG |