Insertion junction: CLIP2.078363_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.078363
Chromosome:chromosome 1
Location:1516188
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre01.g008150 RPK1 RNA-polymerase-subunit kinase; (1 of 1) IPR000719//IPR001932//IPR002290//IPR011009 - Protein kinase domain // PPM-type phosphatase domain // Serine/threonine/dual specificity protein kinase, catalytic domain // Protein kinase-like domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACACACGCACGCACACACACACACAGGTCGTTCACGAGGCGAACATCAC
Internal bar code:ATGGCAGGTATGGACTGCCAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1131
LEAP-Seq percent confirming:27.7778
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:26
LEAP-Seq n unique pos:36

Suggested primers for confirmation by PCR

Suggested primer 1:GAAGTTCCGGACCCCAAGAG
Suggested primer 2:CGTGAACTCGACCACCGTAA