Insertion junction: CLIP2.078375_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.078375
Chromosome:chromosome 7
Location:1455382
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre07.g323750 HAS2 (1 of 1) K06133 - 4'-phosphopantetheinyl transferase (LYS5, acpT); Holo-[acyl-carrier-protein] synthase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGTATCCCCACCTTCCTCTCCAGCCCTACCTCGCTGTCCGCCAGCTGCG
Internal bar code:GCCACGGAATATTCATGCCCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:424
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CGGCTAGTTATTGTGCGTGC
Suggested primer 2:GTTACGGAATCCCCACTGCA