| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.078378 |
| Chromosome: | chromosome 2 |
| Location: | 6723410 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g387467 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCGTCAAAATTGCAAGTACTAACTCAGGGCACCGAGAATCCTCAAATGC |
| Internal bar code: | ATCGGGTCTGAAGGCCGGGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1750 |
| LEAP-Seq percent confirming: | 25.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTACTACTTCGGTGCCCCT |
| Suggested primer 2: | TGTCCTCGACGGTGTTCTTG |