| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.078381 |
| Chromosome: | chromosome 17 |
| Location: | 6622459 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g745547 | (1 of 1) PF05182 - Fip1 motif (Fip1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACGGATTTACAGTGTAGCGCTGACGCCATGCGAGACTGCTGGGACTGTC |
| Internal bar code: | GGGCAGGGAGGAAAGCCGGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2175 |
| LEAP-Seq percent confirming: | 95.7747 |
| LEAP-Seq n confirming: | 68 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 71 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTATTTTGCGTCCGCTTCCG |
| Suggested primer 2: | AAACGCCCCATCCCATTCAT |