| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.078383 |
| Chromosome: | chromosome 12 |
| Location: | 7542549 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g556300 | CFAP300,C11orf70,FBB5 | Flagellar basal body proteome 5 / Dynein assembly factor; (1 of 1) PF14926 - Domain of unknown function (DUF4498) (DUF4498) | 3'UTR |
| Cre12.g556350 | CFAP300 | (1 of 1) PTHR31339//PTHR31339:SF10 - FAMILY NOT NAMED // PECTIN LYASE-LIKE SUPERFAMILY PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGCCCCGGCTTTGCCTCAATGCCTACAAGCCACCTGGACAACTGTTGT |
| Internal bar code: | TTGCCAATCAACTAGTTGGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4194 |
| LEAP-Seq percent confirming: | 76.0 |
| LEAP-Seq n confirming: | 38 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCAGGAGCTTGTGCCTTGA |
| Suggested primer 2: | GCGAGGACTGGTATCGACAG |