| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.078393 |
| Chromosome: | chromosome 9 |
| Location: | 5758560 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g410850 | NAR3,NRT2A | High affinity nitrate/nitrite transporter; (1 of 4) K02575 - MFS transporter, NNP family, nitrate/nitrite transporter (NRT, narK, nrtP, nasA) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGAAGCTTCACACGCCGACCCTGGCGGCGCCAACTGAGATCAACGTGGA |
| Internal bar code: | AATTTCTGGGCAGAGTCGGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1773 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCATGCAAAGAGGATGCG |
| Suggested primer 2: | AAAGGCTGAAGCGTTGCAAG |