| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.078394 |
| Chromosome: | chromosome 7 |
| Location: | 5538935 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g351600 | HEL40 | (1 of 2) PTHR24031:SF303 - DEAD-BOX ATP-DEPENDENT RNA HELICASE 47, MITOCHONDRIAL; possible helicase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCATGCACCTAGGTCCGCGTGCGGGAGCTCTTGAGCGCGTCAGGGCTCA |
| Internal bar code: | CTTTGACTCCCTATGTGCTTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 864 |
| LEAP-Seq percent confirming: | 41.9355 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCTGTGATGCGCTTGATG |
| Suggested primer 2: | ATACTGTAGGGCGGGGTTCT |