| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.078396 |
| Chromosome: | chromosome 1 |
| Location: | 2928635 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g800058 | (1 of 5) PTHR30405 - TRANSPOSASE | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCATTTGGGAAAGTTCGCCCTGTAGTGCTGCTCCAGGTTGGTGAGGAC |
| Internal bar code: | GTGGAGAAGTGGTCGAGCCTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2123 |
| LEAP-Seq percent confirming: | 25.0 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTCGCAGCAATTAGGTTC |
| Suggested primer 2: | GGTGAGTGAAGGTGGGTGAG |