Insertion junction: CLIP2.078408_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.078408
Chromosome:chromosome 6
Location:5377400
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre06.g285300 SUT1 Sugar transporter; (1 of 1) K08200 - MFS transporter, OCT family, solute carrier family 22 (organic cation transporter), member 3 (SLC22A3, OCT3) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAACCTCGTCTCGACTCGCAGCGCCAGCTATACGTGCAACTAGGGCCTC
Internal bar code:GGGGTGTCCCCGGGATCCGTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:482
LEAP-Seq percent confirming:90.625
LEAP-Seq n confirming:29
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:TGTAGCTGCACTCCCCAAAG
Suggested primer 2:GGGTAGGCGTACGGTATGTG