Insertion junction: CLIP2.078411_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.078411
Chromosome:plastome
Location:27635
Confidence (%):63
Locus systematic id Locus common name Defline Feature
CreCp.g802277 2717058,rpl14,ChreCp015 50S ribosomal protein L14; (1 of 1) PTHR11761//PTHR11761:SF13 - 50S/60S RIBOSOMAL PROTEIN L14/L23 // SUBFAMILY NOT NAMED 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATATCTTGTTTAGGTTTACTTTTTTGGATCCTCTGGAAGTTCCAAGAAAG
Internal bar code:CACTATCTCGGTACTCCATCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:52
LEAP-Seq percent confirming:8.33333
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:GCCGGACGTCGTGTTTTAAC
Suggested primer 2:GCGGTACCACTGCCACTAAT