| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.078435 |
| Chromosome: | chromosome 12 |
| Location: | 641840 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g493400 | SMC4 | (1 of 1) K06675 - structural maintenance of chromosome 4 (SMC4); Structural Maintenance of Chromosomes protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGCTCTGCATTCCGTAACCCTTCATGTTGCTGAGAAACGCCCCCAGGG |
| Internal bar code: | CTATCACGATGTTGACATGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 649 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAAGGACGGATTGCAGGTG |
| Suggested primer 2: | CAGCTCCTTCTTGACCTGCA |