| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.078443 |
| Chromosome: | chromosome 1 |
| Location: | 4816476 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g033550 | PDI2 | (1 of 1) PTHR18929:SF87 - PROTEIN C03H12.1, ISOFORM A; Protein disulfide isomerase 2 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGACAAAACCTCAACTGCTTTTTAGATGTGGTCTTTGAAACAAGGATATT |
| Internal bar code: | TGGGTTCTAGCAAGGGAATGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 935 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCATCTGTCACACGTACCC |
| Suggested primer 2: | GAGGGACCAGTAGAGGAGGG |