| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.078448 |
| Chromosome: | chromosome 17 |
| Location: | 2903742 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g802059 | (1 of 3) PF00078//PF00665 - Reverse transcriptase (RNA-dependent DNA polymerase) (RVT_1) // Integrase core domain (rve) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCAACCTGTCTACCGCATACCACCCCCAGACTGATGGGCAAACTGAGCG |
| Internal bar code: | TTGTACGGTGACGTTTTTCAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2015 |
| LEAP-Seq percent confirming: | 45.9459 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCGGTCAATCCTCGATGG |
| Suggested primer 2: | TCATTTCTGCGTGCCACTCT |