| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.078456 |
| Chromosome: | chromosome 1 |
| Location: | 1380900 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g007200 | (1 of 1) K16675 - palmitoyltransferase ZDHHC9/14/18 (ZDHHC9_14_18) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGACCTCCGAAAACCTTTCGGTGTTGGAGAGCGGATGTCCGAAATGCATG |
| Internal bar code: | CCTGTAGGTAAATATGGACTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1680 |
| LEAP-Seq percent confirming: | 87.7193 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 57 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGTCACAGCTCTGCAATGT |
| Suggested primer 2: | CCGTATGCAACCAGTGGTCT |