| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.078464 |
| Chromosome: | chromosome 13 |
| Location: | 1876587 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g575500 | (1 of 726) IPR011009 - Protein kinase-like domain | 5'UTR | |
| Cre13.g801524 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCTATATTAATTTGGCAAGTATTGTAGGCATCCACTTACTAGGCGTTC |
| Internal bar code: | GTTGCGTTACTTCAGCCCCCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2155 |
| LEAP-Seq percent confirming: | 93.75 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGTTCTCCATCATGGCAA |
| Suggested primer 2: | TGCGGTGAGTTGTTTGCATG |