| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.078474 |
| Chromosome: | chromosome 2 |
| Location: | 3121666 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g800210 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATTGCTGCTCGCCACTCCTAACCCCCCGCACTGCCGCTGTCGGCCGCCG |
| Internal bar code: | ATAGCGTCAGATGGATCTATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1791 |
| LEAP-Seq percent confirming: | 41.0959 |
| LEAP-Seq n confirming: | 60 |
| LEAP-Seq n nonconfirming: | 86 |
| LEAP-Seq n unique pos: | 146 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGTTTTTGCGCTCGATGG |
| Suggested primer 2: | TAACCTGCTATTGCGCCCAA |