| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.078482 |
| Chromosome: | chromosome 7 |
| Location: | 725895 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g317550 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAGCTGGGTGTTGGAGGATGATGAGAAGGGCTTGGGGACAGAGGCATT |
| Internal bar code: | GACCCGGACGCCCTAAATATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 626 |
| LEAP-Seq percent confirming: | 41.9355 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTTTGCACCCAGAAACCCA |
| Suggested primer 2: | CAACCCCCTGGCTCATCAAT |