| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.078499 |
| Chromosome: | chromosome 3 |
| Location: | 3653243 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g168500 | (1 of 1) PF15370 - Domain of unknown function (DUF4598) (DUF4598) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTCAAACTCAGCTGCGGGTCGGCTCTGCAAAGTGTGACAAGGAGCAGT |
| Internal bar code: | GGTGGCGGTAGTTCGCGGTCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 195 |
| LEAP-Seq percent confirming: | 4.7619 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCCCGTGAAATGCTGGAG |
| Suggested primer 2: | CCCGTCCGACTCCAAATCAA |