| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.078514 |
| Chromosome: | chromosome 12 |
| Location: | 3461517 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g497350 | PPR13 | PentatricoPeptide Repeat protein 13 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAGACATGTGCTACCATACATCATACGGTAACGCATATTTTGTGTGTGT |
| Internal bar code: | GGTGGTGTGGAAGGCGTCGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 732 |
| LEAP-Seq percent confirming: | 32.2581 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGGCTTCGTTTTGTTGGG |
| Suggested primer 2: | CATACCCCCTCCCACCCATA |