Insertion junction: CLIP2.078531_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.078531
Chromosome:chromosome 16
Location:7462425
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre16.g681578 APC8 (1 of 1) K03355 - anaphase-promoting complex subunit 8 (APC8, CDC23); Anaphase promoting complex subunit 8 5'UTR
Cre16.g681690 (1 of 274) IPR020683 - Ankyrin repeat-containing domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGACACGCTAACCCGAACGGCCGGACCCCACTCATTCTACTTGCCTTTC
Internal bar code:TAGTGTTCTTGTCCTTACTCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:569
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TGCACCTGCTTGGGTTAGAG
Suggested primer 2:AAGGTACTGGGGGTGGTTCT