| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.078580 |
| Chromosome: | chromosome 12 |
| Location: | 3208901 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g500000 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTTGTGGCCATGCGGCCGTCCATGGCGCAACGGTGGTGTGTGAAGACG |
| Internal bar code: | CGCAGTCGGCAGTGAATCTGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3213 |
| LEAP-Seq percent confirming: | 50.0 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTATGGCGTGCTCTTGTGGT |
| Suggested primer 2: | ACAAGTACTTGACTGGGGCG |