| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.078589 |
| Chromosome: | chromosome 6 |
| Location: | 3857327 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278182 | 3'UTR | ||
| lncRNA_TCONS_00179360 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCAACTGGCGGGCCCACCATCTACCTACAACAAATACATAGACAGCTGC |
| Internal bar code: | TGTTTTATAAAATGTAGGATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 415 |
| LEAP-Seq percent confirming: | 5.88235 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGTGTGTGATTGTCGGGA |
| Suggested primer 2: | CAACCAAGTTCCTGTTGCGG |