Insertion junction: CLIP2.078600_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.078600
Chromosome:chromosome 5
Location:280246
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre05.g243353 (1 of 14) PF13540 - Regulator of chromosome condensation (RCC1) repeat (RCC1_2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTTCTGCCTGCGCCTCAGGTCAGCTGCGGCGGCCGCCACAGTCTGGCGC
Internal bar code:TTGGTTAACTGTTCAAACACCC

Confirmation - LEAP-Seq

LEAP-Seq distance:529
LEAP-Seq percent confirming:33.3333
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CTTTAACACACGCACACGCA
Suggested primer 2:ACCATGTGTGTGTCTCGTCC