| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.078608 |
| Chromosome: | chromosome 3 |
| Location: | 8282550 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g199450 | MINE2 | Chloroplast division site determinant | 3'UTR |
| Cre03.g199500 | FAP129 | Flagellar Associated Protein 129 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCGAAGATGGTCACATAAACGCCACACCCTGACAGTCATAAGTCACCC |
| Internal bar code: | GTGGCACTGTGCTGGATAACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2552 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATAAATCGCAGGCGCTCAC |
| Suggested primer 2: | AAACAGATGGCAGGTAGGGC |