| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.078617 |
| Chromosome: | chromosome 8 |
| Location: | 563394 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g359700 | LAS1,LIPA1 | (1 of 2) 2.8.1.8 - Lipoyl synthase / Lipoate synthase; Lipoic acid synthetase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTGCGGGCGCGTGGGCGGTGCATGGGCTTGGTTTGCAGGGGGTGGCAG |
| Internal bar code: | CTCTAGGCCTTCATCAGCTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 140 |
| LEAP-Seq percent confirming: | 5.55556 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTACTTGCCCGAGTCCAA |
| Suggested primer 2: | ACACACACACACACACAGGT |