| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.078619 |
| Chromosome: | chromosome 14 |
| Location: | 3594694 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g630895 | (1 of 1) PTHR13789:SF90 - FAD-BINDING MONOOXYGENASE | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTGTATGGGTAGGTGGGTGGTTGTGTGGGTGTGGGGTGGTGGTGGTTC |
| Internal bar code: | GCGCGTGGAGTCGTTATAGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 167 |
| LEAP-Seq percent confirming: | 7.40741 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTTGGATAGGGTGTGTGG |
| Suggested primer 2: | TGTTTGGGAAAGCACAGGGT |