| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.078645 |
| Chromosome: | chromosome 1 |
| Location: | 3881400 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g025500 | (1 of 1) PTHR19959//PTHR19959:SF135 - KINESIN LIGHT CHAIN // SUBFAMILY NOT NAMED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATAATAGCGGAATTTGTCCCAGAATCCTCAAGGTTGTACACATAGCATT |
| Internal bar code: | ATTTAGCTGTTCGAGGGGTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2609 |
| LEAP-Seq percent confirming: | 21.875 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGGGATTCAGGCTTGACG |
| Suggested primer 2: | CGTTCAAGCCTACGCATTCG |