Insertion cassette: | CIB2 |
Side of cassette: | 5' truncated? |
Strand: | - |
Strain: | CLIP2.078662 |
Chromosome: | chromosome 13 |
Location: | 415405 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre13.g564766 | (1 of 20) 3.1.4.17 - 3',5'-cyclic-nucleotide phosphodiesterase / Cyclic AMP phosphodiesterase | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGCGGGGAAAGCCCAGCGGCGCCATGGCGGTCGGAGTGATCGGGACCC |
Internal bar code: | AGTTGCGGACAGTCCCTCGCCT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1192 |
LEAP-Seq percent confirming: | 98.0392 |
LEAP-Seq n confirming: | 50 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCTCCTGCGAAGGAAGGAAG |
Suggested primer 2: | ACTACCTGCATTGGAAGGCC |