Insertion junction: CLIP2.078663_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.078663
Chromosome:chromosome 5
Location:90558
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre05.g242100 SUB8 (1 of 1) PTHR10795//PTHR10795:SF157//PTHR10795:SF371 - PROPROTEIN CONVERTASE SUBTILISIN/KEXIN // MEMBRANE-BOUND TRANSCRIPTION FACTOR PEPTIDASE, SITE 1 // SUBFAMILY NOT NAMED; Subtilisin-like protease intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCATGGTGTCCACACAATCGCATCCAAACTGCTGCTGCTGCTGCTGCTGC
Internal bar code:TAAATCGGCAAACTCGGCGCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:165
LEAP-Seq percent confirming:30.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:TGTATAGGGGTGGTGGAGGG
Suggested primer 2:AAATGCGCCCACGGTTACTA