| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.078664 |
| Chromosome: | chromosome 16 |
| Location: | 6851495 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g671750 | PFD4 | Prefoldin beta-like protein; (1 of 1) K09550 - prefoldin subunit 4 (PFDN4) | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGAGTGCAGGGGTAGGAGGCGGGCTTCGGCAGTTGGGTCGGTCAGGGG |
| Internal bar code: | CGTAAGGATAGGTGCCCCCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2174 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAAATGCCCCGTGTTGAAG |
| Suggested primer 2: | GTCCGGTCTCCATGCCTATG |