| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.078683 |
| Chromosome: | chromosome 4 |
| Location: | 1443229 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g214100 | CBIX,SIRB1,SIRB | (1 of 1) PF01903 - CbiX (CbiX); Sirohydrochlorin ferrochelatase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGCATATGATACGGTAAGTAGGGCAAAGTGGAGGATTGGGCTCGGGCA |
| Internal bar code: | TATACTATAAGAGTACGGTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 785 |
| LEAP-Seq percent confirming: | 11.7647 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCGTTTTCCTGCATACCA |
| Suggested primer 2: | TAGACGTTCACACCAGCCAC |