| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.078686 |
| Chromosome: | chromosome 3 |
| Location: | 8168595 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g200550 | HYD3 | Iron hydrogenase/prelamin A binding protein; (1 of 1) PTHR11615:SF103 - CYTOSOLIC FE-S CLUSTER ASSEMBLY FACTOR NARFL | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGTGAAGAGCCTGGTGGCGGCGGCCTGGGGCGTCAGTCCGGCGGCCCT |
| Internal bar code: | ATTTCAGAAAGCTAGGCCACTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 640 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCCAGACACACACGTGAT |
| Suggested primer 2: | GTCAGCCTGTGATGGTGTCA |