| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.078687 |
| Chromosome: | chromosome 12 |
| Location: | 1421277 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g488550 | FKM8 | DNA methylase in the FkbM family; (1 of 13) PF05050 - Methyltransferase FkbM domain (Methyltransf_21) | outside_mRNA |
| Cre12.g488600 | (1 of 1) IPR000104//IPR006059 - Antifreeze protein, type I // Solute-binding family 1 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTGGGGAAGTCCGTGTCCATCTCACTGACCATCTCACAGACTAAGCGC |
| Internal bar code: | ACGGTCCGTCAGGTTGTTTAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 367 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATTGCCCCTGAAGCTGTGG |
| Suggested primer 2: | GCATTGACCACCTACCACGA |