| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.078692 |
| Chromosome: | chromosome 5 |
| Location: | 1515084 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234657 | (1 of 1) 2.7.7.87 - L-threonylcarbamoyladenylate synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCTGTGGCTGTTGGCGTCCATGCATGTGCGGCTGTGGCTCTGATTGCG |
| Internal bar code: | GCATGGAATATTGGGACGCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 368 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCAAGGGATTGTGAGCCGT |
| Suggested primer 2: | GGGAAACTTGGTAGGCCTCC |