| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.078704 |
| Chromosome: | chromosome 4 |
| Location: | 1282049 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g214750 | VMP7,VMPL2 | (1 of 2) PTHR21136:SF86 - VESICLE-ASSOCIATED MEMBRANE PROTEIN 7; Endosomal R-SNARE protein, VAMP-like family (R.III) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGTCTTCTGGTGCTGTTTGACTCGCCCGGGTTCAGTTGTCAAGTGATA |
| Internal bar code: | TTTTGTGACAGGTATAAGTCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 879 |
| LEAP-Seq percent confirming: | 89.4737 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGAGCCGCGTTCAGTATA |
| Suggested primer 2: | AGAAAGCCTGTCTTCCTCGC |